Cell Based Assays
Eurofins Discoverx PathHunter eXpress CCR4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0193E2CP0L)
The PathHunter CCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL22/MDC) based activation of CCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR4 and EA-tagged β-Arrestin-2. Activation of CCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter TrkC Bioassay Kit, 10-Plate. (93-0464Y3-00078)
The PathHunter TrkC Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. NT-3) based activation of TrkC receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged TrkC target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Mouse IL-15 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-15 Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 15 (IL-15) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of mouse IL-15 with a lower limit of detection of 1 3 pg/mL and an upper limit of quantification of 10 000 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrators and sample diluent The assay takes approximately 3 5 hours to complete after a 2 hour incubation
Eurofins Discoverx PathHunter eXpress GPR132 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0411E2ACP1M)
The PathHunter GPR132 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR132 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR132 and EA-tagged β-Arrestin-2. Activation of GPR132 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress MC3R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0045E2CP2L)
The cAMP Hunter MC3R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-melanocyte stimulating hormone) based activation of MC3R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MC3R, a naturally Gs coupled GPCR. Upon activation of MC3R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR5 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0402E3CP0M)
The PathHunter SSTR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR5 and EA-tagged β-Arrestin-2. Activation of SSTR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PROKR1 Total GPCR Internalization Assay, 10pl Kit. (93-0764E3CP0L)
The PathHunter PROKR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PROKR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PROKR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PROKR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CSF1R/CSF1R Dimerization Assay, 10pl Kit. (93-1061E3CP5L)
The PathHunter CSF1R/CSF1R Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR150 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0375E2ACP1L)
The PathHunter GPR150 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR150 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR150 and EA-tagged β-Arrestin-2. Activation of GPR150 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human Adiponectin ASY
The Meso Scale Discovery U-PLEX Human Adiponectin Assay Kit quantifies adiponectin in human serum plasma and cell culture supernatants using electrochemiluminescent detection It features MSD s SULFO-TAG labels and multi-array plate technology for high sensitivity and minimal interference The kit includes a 96-well plate with capture antibodies detection antibodies with ruthenium labels calibrators and necessary reagents The assay takes about 3 5 hours facilitating adiponectin measurement in studies on obesity diabetes and cardiovascular disease
Eurofins Discoverx PathHunter eXpress PTGFR U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0936E3CP5M)
The PathHunter PTGFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cloprostenol) based activation of PTGFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGFR and EA-tagged β-Arrestin-2. Activation of PTGFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human M-CSF ASY
The Meso Scale Discovery U-PLEX Human M-CSF Assay is a multiplex immunoassay kit for measuring macrophage colony-stimulating factor (M-CSF) in human serum plasma and cell culture supernatants It uses MSD s electrochemiluminescent detection technology offering high sensitivity up to 5 logs of dynamic range and low sample volume needs The 30-plex kit includes a 96-well plate with capture antibodies detection antibodies and necessary calibrators and controls The workflow is completed in 3 5 hours with a detection limit of 45 pg/mL ideal for accurate M-CSF quantification
Eurofins Discoverx PathHunter Human CFTR-ΔF508 MEM-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS)
The PathHunter Human CFTR-ΔF508 MEM-EA Pharmacotrafficking Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure pharmacochaperone-based rescue of the mutant CFTR's trafficking to the membrane. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human F2RL1 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human F2RL1 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure F2RL1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress MC1R U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0861E3CP5M)
The PathHunter MC1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC1R and EA-tagged β-Arrestin-2. Activation of MC1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.